When the cutoff was determined for the point on the ROC curve that maximizes sensitivity (70.0%) and specificity (76.1%) by Youden’s index, the optimal AFP cut-off value for tumor recurrence was 261.6 ng/mL [AUC = 0.730 (95% CI = 0.619–0.842); P < 0.001], (Fig. 2a). This evidence concerns the gene AFP and neoplasm.