We assessed if cancer cells grown in the bone microenvironment possessed increased Wnt signalling by assessing expression of the Wnt target gene AXIN2. AXIN2 was increased fourfold in MCF-7 cells following CM treatment (p = 0.016), and 40 fold following growth in the bone (p = 0.05) (Fig. 2a). The gene discussed is AXIN2; the disease is cancer.