The specificity of this effect to Wnt signalling was confirmed in MCF-7 cells by dual gene knockdown of the LRP5 and LRP6 receptors using siRNA (Supplementary Fig. 2D, E), and by using breast cancer cells expressing a dominant negative TCF4 (EdTP from ref. 22) (Supplementary Fig. 2F). This evidence concerns the gene LRP5 and breast cancer.