In the subgroup analysis in the present study, although our results showed that FLI1 hypermethylation was significantly more frequent in MSI+ or EBV+ GC than in MSI−/EBV−, FLI1 hypermethylation in plasma was correlated with liver metastasis and a worse 5-year DFS rate in MSI−/EBV− rather than MSI+ or EBV+ GC, which was another novel finding of the present study. Here, FLI1 is linked to gastric cancer.