Previous studies showed that tumor cell death in response to PIT is rapidly induced through membrane damage, which can be detected by the LIVE/DEAD assay.1 The rate of cell death was increased in a NIR light dose‐dependent manner in TROP2‐positive PK‐59, HuCCT‐1, and TFK‐1 cells (Figure 3A‐C). This evidence concerns the gene TACSTD2 and neoplasm.