Apoptosis is mediated by several factors including activation of caspases.25,35 Caspases are involved in apoptosis and have been sub classified by their mechanism of action as initiator caspases such as caspase-8 and -9 or executioner caspases such as caspase-3, -6, and -7.36 To determine whether IL-8 mediated apoptosis requires caspases, we treated mock, caspase-8 and caspase-9 knockdown HCT116 colon cancer cells with TG and Api alone or their combination. This evidence concerns the gene CASP9 and malignant colon neoplasm.