To determine whether specific VEGF isoforms are differentially regulated in neovascular eye disease caused by tissue ischemia, we studied their expression in a mouse model of oxygen-induced retinopathy (OIR), which has the hallmarks of retinopathy of prematurity and proliferative diabetic retinopathy (Figure 2, A and B, and Supplemental Figure 2, A and B) (21, 22). The gene discussed is VEGFA; the disease is proliferative diabetic retinopathy.