Herein, we describe the validation of an anti-C/EBPβ antibody that is reported to detect all three C/EBPβ protein isoforms, but also shows specificity for MYL4, which is present in differentiating myoblasts, myotubes and myofibers, and numerous other cell lines, including commonly used cancer cell lines and cardiac muscle. Here, CEBPB is linked to cancer.