Figure 2B,C demonstrates that in Tks4-KO cells, the E-cadherin signal partly co-localizes with lysosomes. Analyzing the data with Pearson’s correlation showed a correlation coefficient of 0.551 ± 0.120 in the Tks4-KO compared to 0.029 ± 0.014 in the WT. This finding suggests that E-cadherin is partially internalized and found in acidic lysosomes. Western blotting with a second antibody revealed that E-cadherin is indeed downregulated in Tks4-KO cells on the protein level (Figure 2D and Figure S4), thus supporting increased motility in these cancer cells. The gene discussed is SH3PXD2B; the disease is cancer.