Comparatively, at 24 h post-infection, A549 cells that were infected with IAV strain A/Puerto Rico/8/1934 (PR8) at a MOI of 1.0 showed a 7.6 fold increase in IFN-λ2 transcript, a 7.2 fold increase in IL-27 transcript, a 2.5 fold increase in IFN-α2 transcript, and a 2.4 fold increase in IFN-λ1 transcript, but no increase in the IFN-β transcript. This evidence concerns the gene IFNL1 and infection.