Thus, to investigate the independent contribution of IR and NASH that is usually intertwined in patients on miRNAs expression, IR was induced in mice by haploinsufficiency for InsR (InsR+/−) [13,14,15], which determines an impairment of hepatic insulin signaling recapitulating that has been observed in NAFLD patients [8]. This evidence concerns the gene INS and metabolic dysfunction-associated steatohepatitis.