Thus, to investigate the independent contribution of IR and NASH that is usually intertwined in patients on miRNAs expression, IR was induced in mice by haploinsufficiency for InsR (InsR+/−) [13,14,15], which determines an impairment of hepatic insulin signaling recapitulating that has been observed in NAFLD patients [8]. Here, INS is linked to metabolic dysfunction-associated steatotic liver disease.