To determine the clinical diagnosis value of dynamic serum IL-1β, IL-6, IL-8, and TNF-α levels in neonatal sepsis, the prospective study was performed on hospitalizing neonatal sepsis who were classified as culture-proven sepsis (n = 12), as culture-negative sepsis (n = 21), and as healthy newborns (n = 17). The gene discussed is TNF; the disease is Sepsis.