WNV, although used at lower MOI than the other flaviviruses (since infection with MOI 1 led rapidly to complete cell death), induced in the highest levels of innate immune response gene transcripts, especially in NSCs, with a particularly strong stimulation of IFNB1 and the effector IFIT2, a critical mediator involved in antiviral immunity. This evidence concerns the gene IFNB1 and infection.