By 3 days PI, the entire kidney parenchyma in infected WT mice showed severe pyelonephritis (Fig 3B) with invaded growing filaments, pseudohyphae, and yeast cells as shown in a representative GMS stained cortex section (Fig 3A), whereas infected Nos3-/- mice showed comparatively less scattered colonization at 3 days PI (Fig 3A). Here, NOS3 is linked to pyelonephritis.