have shown that EMT and metastatic development are directly linked to the expression of asparagine synthetase and asparagine availability in breast cancer cells, since EMT-associated proteins were shown to have 19% higher asparagine content than total proteome percentage, and knock-down of ATF4, a transcriptional regulator of asparagine synthetase, leads to decreased EMT potential [203]. This evidence concerns the gene ASNS and breast carcinoma.