ATP8B1 and infection: ICP1 produces new progeny virions by 20 min post-infection in PLE(−) cultures, and PLE(+) cultures lyse 20 min post-infection (12), therefore to evaluate total DNA content in infected cells at early, middle and late time points (while avoiding potential DNA loss due to lysis), we collected samples at 4, 8, 12 and 16 min post-infection.