ROR1 and breast carcinoma: However, we did not detect such ROR1–cortactin complexes in lysates prepared from breast-cancer–PDX cells cultured overnight in serum-free media unless they were treated with exogenous Wnt5a (Fig. 1f, g), suggesting that the endogenous Wnt5a was limiting and insufficient to maintain ROR1–cortactin complexes in vitro.