A prospective case-control study was conducted between July 2006 and June 2007 enrolling 100 consecutive GBS cases from Dhaka Medical College Hospital, Bangabandhu Sheikh Mujib Medical University, and Dhaka Central Hospital in Dhaka, Bangladesh. Anti-HEV IgM seroprevalence was significantly higher among GBS patients. A case-control study among GBS patients in Bangladesh documented that 11/100 (11%) had an associated acute HEV infection. IgM seropositive individuals were tested for HEV RNA, yielding one positive serum sample classified as HEV GT1. This evidence concerns the gene CD40LG and Guillain-Barre syndrome.