In this context, the aims of this study were: 1- to produce monoclonal antibodies (mAbs) against S100A8, S100A9 and S100A12 for implementation of ELISA (Enzyme-Linked Immunosorbent Assay) to measure the S100A8/S100A9 complex and S100A12 protein; 2- to compare the plasma levels of these two calgranulins in septic shock patients versus age-matched controls; 3- to compare calgranulins levels during the first 24 h after admission for septic shock between survivors and non-survivors; 4- to evaluate the value of adding calgranulins levels to the initial SOFA score for outcome distribution. Here, S100A8 is linked to septic shock.