We show that Q can differentiate between guide and passenger sequences from the same precursor miRNA duplexes determined by Ago2 IP followed by small RNA sequencing from different cell lines, namely four from human (acute monocytic leukemia THP-126, colon cancer DLD27, colon cancer HCT11628, and T cell leukemia29) and one from mouse (neuroblastoma N2a30). This evidence concerns the gene AGO2 and malignant colon neoplasm.