To determine the influence of vIRF-2 targeting of USP7 on TRAF3 and TRAF6 polyubiquitination in the context of infection, we compared the ubiquitination status of these TRAFs, expressed in tagged form from lentiviral vectors, in lytically reactivated iSLK cells infected with wild-type or vIRF-2.S247A-substituted viruses. This evidence concerns the gene TRAF3 and infection.