Immunofluorescence (IF) analysis of astrocytes (GFAP) and microglia (Iba1) showed increased activation of both cell types surrounding tumor foci, without associated DTX-specific changes, corroborating a well working BM-model [30] and nicely mimicking BM distribution usually observed in patients [31] (Fig. 2b-d). Here, AIF1 is linked to neoplasm.