Furthermore, the present study is unable to disentangle if the numerous DE cell cycle regulation genes identified in the RNA-Seq experiments are relevant to obesity and T2DM or may also be indicative of neoplastic changes, given that the loss of SPRY2 is associated with hepatocarcinogenesis [47–49] and that HepG2 cells themselves are a hepatoma cell line. Here, SPRY2 is linked to hepatocellular carcinoma.