The proportion of Tregs was more closely related to thrombocytopenia, which may be associated with mechanisms that include impaired thrombopoiesis and increased platelet consumption.41 In addition, the use of glucocorticoids and granulocyte colony-stimulating factor (G-CSF) in patients might be able to induce a rapid increase in neutrophils in peripheral blood, which may affect the changes in neutrophils.42 The current study demonstrated the decreased frequencies of Tregs in both BM and PB, and the proportions of Tregs in BM from aGVHD patients were reduced far more than those in PB. The gene discussed is CSF3; the disease is Thrombocytopenia.