Histological analyses of mice nephrotic injury on day 10 showed increased mesangial matrix associated with some degrees of crescent and diffuse mesangial sclerosis (Fig. 3A), decreased Wt1+ podocyte numbers (Fig. 3B,E), and increased glomerular section area (Fig. 3F), compared to the NT and PI treated mice. This evidence concerns the gene WT1 and Diffuse mesangial sclerosis.