To elucidate the role of TAF1 in the proliferation of AE-expressing cells, we knocked down TAF1 in two human t(8;21) AML cell lines, Kasumi-1 and SKNO-1, using two different TAF1 shRNAs that reduce TAF1 expression, compared to a scrambled shRNA control (Fig. 1a, b, middle and right panels). The gene discussed is TAF1; the disease is acute myeloid leukemia.