NADPH oxidase isoform 2 (NOX2) is a type of superoxide generating enzyme complex, which forms ROS and is expressed in myeloid cells, and some cancer cells.15 A study has indicated that the activation of NOX2 blocked autophagic flux by impairing lysosomal enzyme activity.16 In our study, the role of NOX2 in COX7A1‐regulated autophagy was also analyzed, and we found that the level of NOX2 was increased in the COX7A1 Overexpression group (group II) compared with the Control group (group I). The gene discussed is CYBB; the disease is cancer.