However, the results showed that no change in the expression level of BECN1 could be observed after COX7A1 overexpression, suggesting that the cell death induced by COX7A1 overexpression might not be due to autophagy regulation in A549 cells.9 In our research, we mainly analyzed autophagy by detecting the expression level of p62 and LC3, as well as the tandem mRFP‐GFP‐LC3 reporter assay in another human non‐small cell lung cancer cell line, NCI‐H838. This evidence concerns the gene BECN1 and lung cancer.