Furthermore, immobility time in the forced swimming test, which was similarly used as an indicator of depression-like behavior, tended to be lower for HCNP-pp KO mice than controls, but was also not significant in both the time course (time course: p = 0.0686, two-way ANOVA with repeated measures, Figure 5E, left) and total immobility time (Control: 247.5 ± 17.0 s vs HCNP-pp KO: 222.6 ± 21.8 s, p = 0.38, Student’s t-test, Figure 5E, right). Here, PEBP1 is linked to major depressive disorder.