GLI1 and cancer: Since aberrant HH/GLI signaling is linked to increased EMT, invasion and metastasis in various cancers including PDAC [16, 17] we assessed the effect of NT1721-mediated GLI downregulation on the invasive potential of PDAC cells: We used the CytoSelectTM Cell Invasion Assay (Cell Biolabs) to quantify the ability of NT1721-treated PDAC cell lines to migrate through the basement membrane.