Based on the predetermined conditions (Additional file 3: Table S5) — related to breast cancer, related to cancer migration or invasion and proliferation or stemness [20], has not been reported as a miR-133a-3p target, and correlated with poor survival of breast cancer patients (Kaplan-Meier Plotter) — MAML1,a transcriptional coactivator involved in several signaling pathways mainly activating Notch signaling pathway [21], was therefore selected for further experimental verification. Here, MAML1 is linked to cancer.