We used our previously established syngenic orthotopic GBM model in immunocompetent C57BL/6 Thy1-CFP//LysM-EGFP//CD11c-EYFP triple-transgenic animals [19] that recapitulates the typical features of the GBM tumor and is suitable for intravital two-photon imaging analyses. The gene discussed is CFP; the disease is glioblastoma.