In terms of serum AFP concentration, 400 μg/L was selected as the cut‐off point for the diagnosis of liver cancer according to the criteria established by the Chinese Hepatic Cancer Society in Guangzhou in September 2001 (Liu et al., 2010), then the 26 HCC cases showed higher than that of the cut‐off point, with the sensitivity of 56.52%, and the difference was statistically significant compared to the control group (p < .05). The gene discussed is AFP; the disease is hepatocellular carcinoma.