Interestingly, in our study, alisol A-treatment did not significantly increase the number of apoptotic cell death in MDA-MB-231 cells (Figure 2A), and the activation of cleaved-caspases (caspase-3, caspase-8, and caspase-9) was also not observed (Figure 2B), which indicated that the induction of apoptotic cell death is not the main mechanism of alisol A against MDA-MB-231 cancer cells. This evidence concerns the gene CASP9 and cancer.