Excitingly, our experiments using both the Seahorse and Oroboros instruments to measure oxygen consumption and test the function of ETC components revealed increased oxygen consumption and increased complex II function in NCAPD3-deficient HT-29 colon adenocarcinoma cells (Fig. 5; Figs S3 and S4). The gene discussed is NCAPD3; the disease is colon adenocarcinoma.