PITX1 and androgen insensitivity syndrome: Surprisingly, the concentration of ccf mt-DNA between AIS patients and controls was not significantly changed (AIS, 86372,782 ± 18,634,320 GE/ml vs CT, 46,273,967 ± 10,767,971 GE/ml, p = 0.066) (Fig. 1c and Additional file 2).