Under ICC staining, phosphorylated NF-κB p65 subunit usually revealed expression in both cytoplasm and nuclei of the neoplastic cells, but only nuclear signal is suggest to represent the activation of NF-κB because nuclear expression implicates post-translational modification, which is an essential step for cytoplasmic-to-nuclear localization and initiation of transcription [43]. The gene discussed is NFKB1; the disease is intrahepatic cholangiocarcinoma.