The multivariate findings after adjustment for age, body mass index, stage, lymph node metastases, IHC findings (ER, PgR, HER-2, Ki-67 and P53) showed that patients with 25(OH)D levels <16 ng/ml at diagnosis had a significantly higher risk of death (hazard ratio = 2.5-2.9) than the group with 25(OH)D levels ≥16 ng/ml (Table 3). This evidence concerns the gene TP53 and metastatic malignant neoplasm in the lymph nodes.