In this study, we demonstrated that selective gene marker sets comprising 22 genes (Appendix A), such as DSG3, FN1, IGFBP2, CLDN1, HDAC7, and L1CAM as single gene marker candidates, are useful for predicting the prognosis of glioblastoma, based on the status assessment of EMT and GSCs that can be developed as novel target candidates for therapeutic intervention. This evidence concerns the gene DSG3 and glioblastoma.