Furthermore, using a genetically inducible neural stem cell-specific reporter mouse line (nestin-CreERT2-R26R-YFP), which allows the labelling and tracking of neural stem cell proliferation, survival, and differentiation in ischemic brain, it was shown that post-stroke treatment with an Shh pathway agonist increases survival of newly born cells derived from both subventricular and subgranular zones and neurons in the ischemic brain [59]. This evidence concerns the gene SHH and stroke disorder.