As shown in Figure 1c,d, Western blot analysis of whole-cell lysates from MCF10A, MCF7, and MDA-MB-231 cells with a specific anti-AC8 antibody revealed that this protein is scarcely expressed in the non-tumoral cell line, while it is highly expressed in MCF7 and MDA-MB-231 breast cancer cells. The gene discussed is ADCY8; the disease is breast cancer.