The Orai1 and AC8 expression normalized to the β-actin content indicates that Orai1 expression was 371 ± 12 and 393 ± 22% of that in MCF10A cells in MCF7 and MDA-MB-231 cells, respectively, while the AC8 expression was 611 ± 75 and 621 ± 98% of that in MCF10A cells in MCF7 and MDA-MB-231 cells, respectively; therefore, the quantitative analysis indicated that AC8 overexpression in breast cancer cells is significantly greater than that of Orai1. This evidence concerns the gene ACTB and breast cancer.