We evaluated GLS2 and GLS expression levels in additional cell lines and found that GLS2 expression was reduced in mesenchymal breast cancer cell lines (e.g., SUM159, MDA231, and MDA 468) relative to the epithelial breast cancer cell line (MCF7) and that GLS expression was enhanced (Supplementary Figure S1A–C). This evidence concerns the gene GLS2 and breast cancer.