Consistent with the western blot analysis, mio, mei-w68 double mutant ovarian cysts have an approximately three-fold increase in p4E-BP staining in region 2b of the germarium relative to mio mutants (Figure 2C,E and H) Notably, the increase in TORC1 activity in the mio, mei-w68 double mutants is restricted to the germline, consistent with blocking meiotic DSBs having cell autonomous effects on TORC1 activity in the germline. This evidence concerns the gene CRTC1 and Ovarian cyst.