TLR4 and intestinal neoplasm: Ripk3 signalling promotes intestinal tumours by up‐regulating cytokines IL23 and IL1β, which are required for expanding IL‐17‐producing T cells through I‐MDSCs, a distinct MDSC subset that is dependent on GM‐CSF.37 Ripk3 activity is mediated by TLR4.38 We ever used CD11b/Gr1 double staining to detect the expression of MDSC in spleen and bone marrow of WT, ApcMin/+, ApcMin/+TLR4−/− mice by flow cytometry.