To investigate the effect of ACAT1 on the viability and proliferation of ccRCC cells, ACHN and Caki1 cells were treated with lentiviral control and lentiviral ACAT1 for 48 h and determined by MTT assay and cloning formation assay, suggesting that overexpression of ACAT1 drastically restrained ccRCC cell proliferation (Figures 7A–E). Here, ACAT1 is linked to nonpapillary renal cell carcinoma.