The aim of this retrospective review was to characterize the clinico-pathological features, including age at diagnosis, stage at presentation, estrogen receptor (ER), progesterone receptor (PR), and Human Epidermal Growth Factor Receptor2 (Her-2/neu) of breast cancer pathology specimens reviewed at the National Health Laboratory (NHL) and Diagnofirm Medical Laboratory (DML) in Gaborone, Botswana. The gene discussed is ESR1; the disease is breast cancer.