To better understand whether BCG-Denmark may result in a more mature antigen-stimulated CD4+ T cell memory differentiation, which along with greater polyfunctionality provides important insight into protective responses against infections (29–32), we compared the memory phenotypes of the total cytokine BCG responding cells (i.e., cytokine positive) defined as naïve (CD27+CD45RA+), early differentiated (ED, CD27+CD45RA–), late differentiated (LD, CD27–CD45RA–) and terminally differentiated (TD, CD27–CD45RA+) CD4+ T cells. The gene discussed is CD27; the disease is infection.