Due to the prevalence of the EWS-FLI1 oncoprotein in ES and its crucial role as an ES biomarker,5,30 we first detected the putative role of EWS-FLI1 in promoting TNC transcription through bioinformatics analysis and further explored this role by the dual-luciferase reporter and ChIP assays. The gene discussed is EWSR1; the disease is Ewing sarcoma.