In the current work, we discovered the following: (1) the expression of TNC in ES tumour tissue and cells, (2) the prognostic role of TNC in predicting ES patient survival, (3) the pro-tumorigenic role of TNC in ES cell lines (in vitro) and nude mice (in vivo), (4) the transcriptional role of the EWS-FLI1 oncoprotein in promoting TNC expression, (5) the expression of MALAT1 in ES specimens, and (6) the connection between TNC and MALAT1 and their underlying mechanism in promoting ES tumour progression. This evidence concerns the gene EWSR1 and Ewing sarcoma.