A CCK8 assay to assess A673 and SKNMC cell viability showed that the proliferation rates of the TNC-ko groups were significantly lower than those of the TNC-wt groups (p < 0.01) and that the addition of exogenous TNC failed to rescue tumour cell growth in both the TNC-wt and TNC-ko groups (Fig. 2b). The gene discussed is TNC; the disease is neoplasm.