To simulate DKD in vitro, we incubated podocytes with different concentrations of AGE-BSA (advanced glycation end products-bovine serum albumin) (50, 100, and 200 μg/ml) for 12 h or treated with 100 μg/ml AGE-BSA for different times (3, 6, 12, 24, and 36 h). This evidence concerns the gene ALB and diabetic kidney disease.