NSD2 and Miyoshi myopathy: In order to address this question, we used two previously characterized isogenic cell lines generated from the patient-derived KMS11 t(4;14) MM cell line: non-translocated knockout (NTKO) and translocated knockout (TKO) cells, which have the translocated allele or the endogenous NSD2 allele, respectively inactivated by insertion of a stop codon just after exon 6, leading to a truncated protein that lacks all functional domains (Fig. 1a)24.