Compared with the migraine-VEH group, NTG-treated animals showed a significant increase or decrease in the following peptides in different regions (with full name and UniProt accession numbers in parenthesis): DH - CGRP ([83–119]) (Calcitonin gene related peptide1 Q99JA0) and SCGI [356–374] (Secretogranin-1 P16014), PAG - PENK A[197–208] (Pro-Enkephalin A- P22005), TG - HBA1[111–142] (Hemoglobin Subunit alpha1 Q91VB8) and MCTP4[208–228] (Mast cell protease-4 P21812), NAc - CLTA[181–204] (Clathrin light chain A- O08585) and TUBB5 [137–167] (Tubulin beta-5 P99024) (Fig. 3A). This evidence concerns the gene PENK and migraine disorder.