To determine the impact of treatments on CRC cell cycle progression and proliferation, we treated each CRC cell line with 10μM FND-4b, 5μM PI-103 and 100nM SN-38 as mono- or dual-therapy regimens for 24h and analyzed the protein expression of cyclin D1 by western blot. This evidence concerns the gene CCND1 and colorectal carcinoma.